High levels of fatty acid-binding protein 5 excessively enhances fatty acid synthesis and proliferation of granulosa cells in polycystic ovary syndrome

Background Polycystic ovary syndrome (PCOS) is one of the most complex endocrine disorders in women of reproductive age. Abnormal proliferation of granulosa cells (GCs) is an important cause of PCOS. This study aimed to explore the role of fatty acid-binding protein 5 (FABP5) in granulosa cell (GC) proliferation in polycystic ovary syndrome (PCOS) patients. Methods The FABP5 gene, which is related to lipid metabolism, was identified through data analysis of the gene expression profiles of GSE138518 from the Gene Expression Omnibus (GEO) database. The expression levels of FABP5 were measured by quantitative real-time PCR (qRT‒PCR) and western blotting. Cell proliferation was evaluated with a cell counting kit-8 (CCK-8) assay. Western blotting was used to assess the expression of the proliferation marker PCNA, and immunofluorescence microscopy was used to detect Ki67 expression. Moreover, lipid droplet formation was detected with Nile red staining, and qRT‒PCR was used to analyze fatty acid storage-related gene expression. Results We found that FABP5 was upregulated in ovarian GCs obtained from PCOS patients and PCOS mice. FABP5 knockdown suppressed lipid droplet formation and proliferation in a human granulosa-like tumor cell line (KGN), whereas FABP5 overexpression significantly enhanced lipid droplet formation and KGN cell proliferation. Moreover, we determined that FABP5 knockdown inhibited PI3K-AKT signaling by suppressing AKT phosphorylation and that FABP5 overexpression activated PI3K-AKT signaling by facilitating AKT phosphorylation. Finally, we used the PI3K-AKT signaling pathway inhibitor LY294002 and found that the facilitation of KGN cell proliferation and lipid droplet formation induced by FABP5 overexpression was inhibited. In contrast, the PI3K-AKT signaling pathway agonist SC79 significantly rescued the suppression of KGN cell proliferation and lipid droplet formation caused by FABP5 knockdown. Conclusions FABP5 promotes active fatty acid synthesis and excessive proliferation of GCs by activating PI3K-AKT signaling, suggesting that abnormally high expression of FABP5 in GCs may be a novel biomarker or a research target for PCOS treatment. Supplementary Information The online version contains supplementary material available at 10.1186/s13048-024-01368-6.


Introduction
Polycystic ovary syndrome (PCOS) has a prevalence of 5-10% and has become one of the most complex endocrine disorders in women of reproductive age, seriously affecting women's reproductive health and quality of life [1].The clinical features of PCOS include hyperandrogenism, insulin resistance, polycystic ovarian morphology, and ovulatory dysfunction [2].To date, the pathogenesis of PCOS has not been elucidated.
Granulosa cells (GCs) are an important somatic component of the ovary that surrounds oocytes and interacts with them to provide nutritional and mechanical support.Additionally, these cells maintain follicular development and the ovarian microenvironment through the process of follicle maturation [3].The normal proliferation and functional transformation of GCs play key roles in the transition of primordial follicles to mature follicles [4].Studies have shown that certain molecules highly expressed in PCOS GCs effectively promote the proliferation of GCs [5,6].Moreover, ovarian GCs in patients with PCOS exhibit abnormal and excessive proliferation, which is associated with abnormal follicular development and ovulation in polycystic ovaries [7,8].As a result, inhibiting GC proliferation may be a potential treatment for PCOS [9].PI3K-AKT signaling is a fundamental pathway that regulates GC proliferation and apoptosis during follicular development [10][11][12].p-AKT promotes cell survival, growth, and proliferation by phosphorylating the downstream target FOXO1 to inhibit transcription [13], and recent studies have shown that PI3K-AKT signaling is abnormal in PCOS GCs [14,15].Overall, abnormal and excessive proliferation of GCs may be involved in the pathogenesis of PCOS, and studying the underlying mechanism may provide new insights for the development of novel therapeutic targets for PCOS.
As an endocrine disorder regulated by hormones, PCOS exhibits certain metabolic abnormalities, such as impaired metabolism of steroid hormones and insulin [16][17][18].Diverse pathways that are associated with PCOS-related metabolic abnormalities, including pathways related to fatty acid synthesis and metabolism, have been identified.Compared with healthy control individuals, PCOS patients exhibit significant lipid metabolism disorders [19][20][21].FABP5, an intracellular carrier of longchain fatty acids and related active lipids, such as endocannabinoids, regulates the metabolism and activity of its ligands and selectively transfers specific fatty acids from the cytoplasm to the nucleus, where it activates nuclear receptors [22,23].Although the relevant literature shows that FABP5 can effectively promote fatty acid synthesis and metabolism and thus regulate cell proliferation, the regulatory effect of FABP5 on the proliferation of GCs in PCOS patients has not been reported.
In this study, we investigated whether FABP5 plays an important role in regulating GC proliferation and fatty acid synthesis and metabolism by activating PI3K-AKT signaling.Our results emphasize that a high level of FABP5 in the GCs of PCOS patients leads to excessive proliferation and fatty acid accumulation in these cells, which may be an underlying mechanism of excessive GC proliferation in PCOS patients and could lead to an effective strategy for the clinical diagnosis and treatment of PCOS.

Data processing of SEGs (specifically expressed genes) and DEGs
The long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) in the original data were removed.P values < 0.05 and a |log2-fold change (FC)|≥ 1 was set as the cutoff criteria for obtaining candidate significantly expressed genes; those significantly expressed genes whose expression value was 0 in the PCOS group or control group were considered SEGs, and the remaining significantly expressed genes were considered SEGs.A follow-up analysis was subsequently conducted on the DEGs and SEGs.

Gene Ontology and KEGG pathway analyses of DEGs
The DEG ensemble IDs were translated into official gene symbols using DAVID (https:// david.ncifc rf.gov/ home.jsp).Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were also conducted using the online analysis software Shanghai Biotechnology Corporation (http:// enrich.shbio.com).A corrected P value < 0.05 indicated significant gene enrichment.

Patients and sample collection
Informed consent was obtained from all participants prior to undergoing in vitro fertilization (IVF) or embryo transfer procedures due to sperm quality issues or tubal obstructions, and use of human GCs were approved by the ethics committee of Nanjing Drum Tower Hospital on 5 December 2013 (2013-081-01).Human ovarian GC samples were obtained at the Reproductive Center of the Drum Tower Hospital Affiliated with Nanjing University Medical School from May 2018 to May 2019.The diagnostic criteria for PCOS were based on the 2003 Rotterdam criteria [2].Those who ovulated normally, including those with infertility due to tubal or male factors, were also recruited as a control group.The GCs were isolated from normal donors (n = 12, range, 24-30 years old) or from PCOS donors (n = 12, range, 22-29 years old); these procedures were approved by the institutional review board of the Drum Tower Hospital of Nanjing University on 5 December 2013 (2013-081-01).The collected human GCs were properly stored for use in subsequent experiments.

Mice
All mouse experiments involved in this study were approved by the Institutional Animal Care and Use Committee of Nanjing Drum Tower Hospital (SYXK 2019-0059).GCs were isolated from 3-week-old ICR female mice purchased from Nanjing Medical University (Nanjing, China).All mice were maintained in the Animal Laboratory Center of Nanjing Drum Tower Hospital (Nanjing, China) with a 12/12 h light/dark cycle (lights off at 1900 h) and food and water available ad libitum.

Vector construction, small interfering RNA (siRNA) synthesis, and transfection
The FABP5 CDS was amplified by using the following primers: FABP5-Forward: 5'-ACT TGA ATT CAA TGG CCA CAG TTC AGC AGC TG-3' and FABP5-Reverse: 5'-ATA GTT CTA GAG AAC TGA GCT TGG TCA TTC TC-3' .The amplicons were subcloned and inserted into the pCS2-myc plasmid by using the restriction enzymes EcoR I and Xba I.The PCR procedure was as follows: 95 °C for 3 min; 5 cycles of 95 °C for 15 s and 58 °C for 30 s; 5 cycles of 95 °C for 15 s and 56 °C for 30 s; 5 cycles of 95 °C for 15 s and 54 °C for 30 s; 25 cycles of 95 °C for 15 s and 52 °C for 30 s; and 52 °C for 5 min; and a hold at 16 °C.The selected monoclonal bacteria were sequenced (Bioengineering Co., Ltd., Shanghai, China) and confirmed to be correct for plasmid extraction.FABP5 siRNA and negative control (NC) siRNA were synthesized by Guangzhou RiboBio Co., Ltd.The FABP5 siRNA sequences used were as follows: siFABP5-Forward: 5'-UGG GAA GGA AAG CAC AAU AUU-3' and siFABP5-Reverse: 5'-UAU UGU GCU UUC CUU CCC AUU-3' .
Prior to transfection, KGN cells (1 × 10 5 cells/well) were plated in a 6-well plate with complete medium and incubated overnight.FABP5 siRNA and the pCS2-myc-FABP5 plasmid were transfected into KGN cells with Lipofectamine ™ 3000 Transfection Reagent (Thermo Fisher Scientific, Shanghai, China).The groups transfected with the negative control siRNA, FABP5 siRNA, empty vector control or pCS2-myc-FABP5 plasmid were referred to as the siNC, siFABP5, Ev-ctrl and myc-FABP5 groups, respectively.The transfection procedure was performed according to the manufacturer's instructions.After 48 h, the transfected KGN cells were used in subsequent experiments.

qRT-PCR verification
Total RNA was extracted from cells after treatment with TRIzol (Ambion/Thermo Fisher Scientific, Shanghai, China) according to the manufacturer's instructions.The concentration and purity of all the RNA were tested after extraction, and the A260/A280 values were greater than 2.0.Reverse transcription was performed to generate cDNA using 5X All-In-One RT MasterMix (with AccuRT Ge5X All-In-One RT MasterMix and AccuRT Genomic DNA Removal Kit) (Applied Biological Materials, Vancouver, Canada) for qRT-PCR to detect FABP5, ACSL1, GPAM, LPIN1 and DGAT2 mRNA expression in human GCs and KGN or Fabp5 mRNA in mouse ovarian tissues, which was performed according to the instructions provided with ChamQ SYBR qRT-PCR Master Mix (without ROX) (Vazyme, Nanjing, China) using the fluorescence reagent SYBR and a qTOWER 3 G touch instrument (Analytik Jena, Jena, Germany).The data were analyzed by using the 2 −ΔΔCt relative quantitative method with human or mouse 18S rRNA as an internal control in Microsoft Excel software.The primers used are listed in Table 1.

Cell proliferation assay
Transfected KGN cells (1 × 10 4 cells/well) were plated in a 96-well plate for 24, 48 or 72 h.A total of 10 µl of Cell Counting Kit-8 (CCK-8) reagent (Dojindo, Kyushu, Japan) was then added to each well, and the cells were incubated at 37 °C for 1 h.The optical density (OD) at 450 nm was subsequently measured.

Lipid droplet staining
KGN cells were grown on 24-well plates, washed three times with PBS and fixed with 10% paraformaldehyde (PFA) at room temperature for 30 min.The fixed KGN cells were washed three times with PBS, incubated with Nile Red (1 mg/ml) (Macklin, Shanghai, China) for 10 min at room temperature and subsequently stained with DAPI for 5 min at room temperature.Images were obtained overnight in the dark with a fluorescence microscope (LEICA DM3000 LED, Wetzlar, Germany).

Immunohistochemistry
Paraffin-embedded ovarian tissues from PCOS and normal mice were obtained based on Li's work [25].Tissue sections were dewaxed and treated with freshly prepared PBS containing 0.3% hydrogen peroxide for 10 min to block endogenous peroxidase activity.Antigen retrieval was conducted by autoclaving the samples at 121 °C for 15 min in the presence of EDTA (pH = 9.0), followed by incubation in blocking solution to block endogenous biotin for 30 min.The sections were washed with TBS and then stained overnight with the primary antibody FABP5 (1:500; Proteintech, Chicago, USA) at 4 °C.Subsequently, the sections were rinsed with PBS and incubated with an HRPconjugated goat anti-rabbit secondary antibody (rabbit ABC detection kit, ZSBio, Beijing, China) at 37 °C for 30 min.Next, the sections were stained with 3,3'-diaminobenzidine (DAB) and counterstained with hematoxylin.Control sections were prepared concurrently with the experimental sections and treated with nonspecific rabbit IgG.Nonspecific staining was not detected in the controls.

Immunofluorescence staining
KGN cells or human primary ovarian GCs were grown in 24-well plates that were prepositioned with plate inserts and fixed with 4% (w/v) PFA for 30 min at room temperature.Then, the cells were permeabilized with 0.5% (v/v) Triton X-100/PBS for 15 min, followed by blocking with 3% (g/v) BSA/PBS.Subsequently, the KGN cells were incubated overnight with the primary antibody Ki67 (1:2000; Abcam, Shanghai, China) or FABP5 (1:500; Proteintech, Chicago, USA) at 4 °C.The next day, the KGN cells were incubated with a goat anti-rabbit secondary antibody (Alexa Fluor 594) (1:1000; Abcam, Shanghai, China) at room temperature for 60 min, after which the nuclei were stained with DAPI.Finally, the KGN cells were visualized overnight in the dark with a fluorescence microscope (LEICA DM3000 LED, Wetzlar, Germany).

Statistical analysis
All the data are presented as the means ± SEMs.Statistical significance was evaluated with a two-tailed unpaired t test.A 2-tailed P < 0.05 was considered to indicate statistical significance.All the statistical analyses were implemented using GraphPad Prism 9 statistical software.

Identification of significantly differentially expressed genes (DEGs)
To determine the important regulatory genes in GCs of PCOS patients, we downloaded the gene expression profiles of GSE138518 from the GEO database in a journal article published by Mao [24].
After removing the related interfering noncoding RNAs and genes that were not significantly expressed, we obtained all DEGs and SEGs with P values < 0.05 and |log2-FC|≥ 1 for a subsequent cluster analysis, and the results showed that differences occurred between the two samples, thus confirming that the dataset met the conditions for differential expression analysis (Fig. 1A,  B).Subsequently, GO function and KEGG pathway analyses were performed, and we found that the DEGs and SEGs were enriched in 26 biological process (BP) terms, 3 cellular component (CC) terms and 1 molecular function (MF) term (Fig. 1C, Supplementary Table 1).KEGG pathway analysis of the DEGs and SEGs revealed that they were enriched mainly in histidine metabolism, propanoate metabolism, the TCA cycle, and the proteasome (Fig. 1D, Supplementary Table 2).
Patients with PCOS often exhibit abnormalities in lipid metabolism.Therefore, we focused particularly on GO functional terms and pathways related to lipid metabolism (fatty acid binding, fatty acid degradation, and fatty acid metabolism) (Fig. 1C, D).According to the analysis of the database, 6 hub DEGs (ACADM, ALDH7A1, FABP5, STX3, ACAT1, and HACD4) were highly expressed in PCOS GCs, and 2 hub DEGs (PTGDS and OXER1) were highly expressed in normal GCs (Fig. 1E).

High levels of FABP5 are present in the GCs of patients and mice with PCOS
FABP5 was chosen for investigation because its expression in PCOS GCs was 2.3-fold greater than that in normal GCs.As an important intracellular carrier of fatty acid metabolism, FABP5 is likely involved in the occurrence of PCOS.To clarify this hypothesis, we collected GCs from PCOS patients and normal patients to detect FABP5 expression and found that FABP5 mRNA and protein were expressed at high levels in the GCs of PCOS patients compared with those of normal patients (Fig. 2A,  B) (A: Normal vs. PCOS = 1.044 ± 0.308 vs. 1.567 ± 0.341, P < 0.01).Furthermore, FABP5 expression in the ovarian tissues of PCOS mice was significantly greater than that in the ovarian tissues of normal mice (Fig. 2C, D, E, F) (C: Normal vs. PCOS = 1.104 ± 0.500 vs. 2.351 ± 0.376, P < 0.01; E: Normal vs. PCOS = 0.530 ± 0.148 vs. 1.007 ± 0.062, P < 0.05).Importantly, high expression of FABP5 was more obvious in GCs and oocytes at all follicle levels in PCOS mouse ovaries (Fig. 2F).

Discussion
Although the current clinical management and treatment strategies for PCOS include healthy lifestyles and drug interventions, such as metformin, the alpha-glycosidase inhibitor acarbose, and short-term contraceptives, they mainly mitigate metabolism and endocrine dysfunction only in patients with PCOS [28][29][30][31].Moreover, these nonsurgical treatments have limited effectiveness and require considerable time, and the molecular mechanisms underlying metabolism and endocrine disorders in PCOS patients have not been elucidated.
The primary aim of this study was to explore whether abnormal expression of FABP5 in the GCs of PCOS patients is related to the excessive proliferation and impaired fatty acid synthesis of GCs in PCOS patients and to the underlying mechanism involved.Our results demonstrated that FABP5 was upregulated in the GCs of PCOS patients and in those of PCOS mice.In vitro, FABP5 overexpression promoted the proliferation and fatty acid synthesis of KGN cells.In addition, PI3K-AKT signaling was promoted by FABP5 overexpression in KGN cells, whereas the PI3K-AKT inhibitor LY2940002 significantly suppressed the proliferation-and fatty acid synthesis-promoting effects induced by FABP5 overexpression.In contrast, FABP5 knockdown inhibited PI3K-AKT signaling, and the PI3K-AKT agonist SC79 significantly rescued the inhibition of KGN cell proliferation and lipid droplet formation caused by FABP5 knockdown.
PCOS patients with hyperandrogenaemia exhibit significantly abnormal lipid metabolism [32][33][34].As a small and highly conserved cytoplasmic carrier that regulates lipid metabolism, increased FABP5 expression was observed in PCOS GCs.In addition, we used KGN cells, a steroidogenic human granulosa-like tumor cell line that maintains physiological characteristics similar to those of human immature GCs [35,36], to verify the function of FABP5 in vitro and found that FABP5 effectively promoted the generation of fatty acids in KGN cells.Studies have shown that FABP5 contributes to fatty acid synthesis [26,[37][38][39].A previous study showed that serum adipocyte-FABP levels were associated with metabolic abnormalities in PCOS patients [40], while another study indicated that adipocyte-FABP levels were likely associated with obesity in women with PCOS rather than with hyperandrogenaemia [41].These studies undoubtedly support the positive effect of FABP5 on lipid formation in KGN cells.
At present, the pathogenesis of PCOS has not been described in detail.Numerous studies have shown that hypoproliferation and severe apoptosis of ovarian GCs are obvious phenotypes in PCOS patients [19,42].However, there are also some studies with a different view; they believe that PCOS patients have excessive proliferation of GCs [5][6][7][8].Therefore, further studies are needed to elucidate the underlying mechanisms involved.Although studies have shown that FABP5 also contributes to proliferation [26,27,43] in various other cell types, investigations of FABP5 in the context of PCOS are relatively limited.In our study, we observed that FABP5 could significantly promote KGN cell proliferation, suggesting that FABP5 might lead to the pathogenesis of PCOS through excessive proliferation of GCs.This is the first study to confirm that FABP5 is involved in promoting fatty acid synthesis and cell proliferation, which suggests that an excess of FABP5 in GCs may result in excessive fatty acid accumulation and proliferation of GCs, possibly leading to PCOS.
FABP5 has been reported to be involved in regulating cell proliferation and lipid metabolism by activating the PI3K-AKT signaling pathway [27,44].PI3K-AKT signaling is the fundamental signaling pathway that regulates the proliferation and apoptosis of GCs during follicular development, and changes in PI3K-AKT signaling may be a key factor in the pathogenesis of PCOS [45].Consistent with the above conclusion, overexpression or knockdown of FABP5 effectively increased or decreased AKT phosphorylation, respectively, which resulted in abnormal proliferation of GCs.In addition, as with GC proliferation, we also found that fatty acid synthesis in KGN cells is affected by AKT phosphorylation.Despite these findings, studies indicating the regulation of fatty acid synthesis by PI3K-AKT signaling in PCOS are limited.Several reports have shown that PI3K-AKT signaling is involved in regulating glucose metabolism in PCOS patients [45,46].In summary, our work is the first to demonstrate that FABP5 may excessively promote GC proliferation and fatty acid synthesis through excessive activation of PI3K-AKT signaling.
This is an innovative study that revealed that a high level of FABP5 causes excessive proliferation and impaired fatty acid synthesis in GCs and may be an underlying mechanism of PCOS.However, there are several potential limitations in our study.For example, many PCOS patients are needed to further validate the expression of FABP5.In addition, investigating FABP5-interacting molecules is conducive to exploring the pathogenesis of PCOS.However, whether FABP5 can lead to the progression of PCOS through the activation of PI3K-AKT signaling, which affects the proliferation of granular cells, needs further verification.A deeper understanding of these molecules could lead to the development of targeted drugs that can be used in effective clinical treatment to alleviate or treat the onset of PCOS, and this goal will be part of our future research.

Fig. 1
Fig. 1 Bioinformatic analysis of DEGs with significant differences in the GSE138518 dataset.A Volcano plot filtering of the SEGs and DEGs with significant differences in the GSE138518 dataset.B Cluster heatmap of significant DEGs.C Top 30 enriched GO terms of the significant DEGs.D The 30 pathways associated with the significant DEGs.E Cluster heatmap of the key significant DEGs involved in lipid metabolism

Fig. 2
Fig. 2 Aberrantly increased expression of FABP5 in GCs of patients and ovaries of mice with PCOS.A Bar plot showing FABP5 mRNA expression in the human primary ovarian GCs of PCOS patients and healthy individuals, as measured by qRT-PCR (n = 12).B Immunofluorescence staining of FABP5 in human primary ovarian GCs of PCOS patients and healthy individuals.Scale bar, 50 μm.C Bar plot showing the expression of Fabp5 mRNA in the ovaries of mice with PCOS measured by qRT-PCR (n = 6).D, E Immunoblot plot (D) and bar plot of the statistical analysis (E) showing the expression of FABP5 in the ovaries of mice with PCOS determined by Western blot analysis (n = 4).F Immunohistochemical plot of FABP5 expression and localization in the ovaries of mice with PCOS.A two-tailed unpaired t test was used for all the statistical analyses in this section.** P < 0.01

Fig. 3
Fig. 3 Overexpression of FABP5 promotes fatty acid synthesis in KGN cells.A Bar plot showing the expression of FABP5 in KGN cells after transfection with the PCS2-myc-FABP5 or empty vector plasmids for 48 h, as determined via qRT-PCR.B, C Immunoblotting plot (B) and bar plot of statistical analysis (C) showing the expression of FABP5 in KGN cells transfected with PCS2-myc-FABP5 or empty vector plasmids for 48 h.D Nile red staining of KGN cells after 48 h of FABP5 overexpression.The red signal indicates the formation of lipid droplets.All the samples were also stained with DAPI.Scale bar, 100 μm.E Statistical analysis of the number of lipid droplets.Five fields of view were randomly selected.F Bar plot showing the ACSL1, GPAM1, LPIN1 and DGAT2 mRNA expression in KGN cells after 48 h of FABP5 expression.A two-tailed unpaired t test was used for all the statistical analyses in this section.* P < 0.05, ** P < 0.01, ***P < 0.001.The groups transfected with the empty vector control or pCS2-myc-FABP5 plasmids were referred to as the EV-ctrl and myc-FABP5 groups, respectively

Fig. 4
Fig. 4 Knockdown of FABP5 inhibits fatty acid synthesis in KGN cells.A Bar plot showing the expression of FABP5 in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h.B, C Immunoblot plot (B) and bar plot of the statistical analysis (C) showing the expression of FABP5 in KGN cells transfected with FABP5 or negative control siRNAs for 48 h.D Nile red staining diagram of KGN cells after transfection with FABP5 siRNA for 48 h.The red signal indicates the formation of lipid droplets.All the samples were also stained with DAPI.Scale bar, 100 μm.E Statistical analysis of the number of lipid droplets.Five fields of view were randomly selected.F Bar plot showing ACSL1, GPAM1, LPIN1 and DGAT2 mRNA expression in KGN cells after transfection with FABP5 siRNA for 48 days.A two-tailed unpaired t test was used for all the statistical analyses in this section.* P < 0.05, ** P < 0.01, ***P < 0.001.The groups transfected with the negative control siRNA or with FABP5 siRNA were referred to as siNC and siFABP5, respectively

Fig. 5 FABP5
Fig. 5 FABP5 facilitates KGN cell proliferation.A Immunoblotting plot showing the expression of FABP5 and the cell proliferation marker PCNA in KGN cells after 48 h of FABP5 overexpression.B A line chart showing the proliferation of KGN cells after 48 h of FABP5 overexpression, as determined by CCK8 assays.C Immunofluorescence staining of Ki67 in KGN cells after 48 h of FABP5 overexpression.All the samples were also stained with DAPI.Scale bar, 100 μm.D Statistical analysis of the Ki67-positive cells.Five fields of view were randomly selected.E Immunoblotting plot showing the expression of FABP5 and PCNA in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h.F Line chart showing the proliferation of KGN cells after transfection with FABP5 or negative control siRNAs for 48 h, as determined by CCK8 assays.G Immunofluorescence staining of Ki67 in KGN cells after transfection with FABP5 or negative control siRNAs for 48 h.All samples were also stained with DAPI.Scale bar, 100 μm.H Statistical analysis of the Ki67-positive cells.Five fields of view were randomly selected.A two-tailed unpaired t test was used for all the statistical analyses.* P < 0.05, ** P < 0.01, ***P < 0.001

Fig. 6 FABP5
Fig. 6 FABP5 accelerates the proliferation of KGN cells by activating PI3K-AKT signaling.A Immunoblotting plot showing the expression of FABP5 and AKT and the phosphorylation of AKT in KGN cells after 48 h of FABP5 overexpression or knockdown.B, C Line chart showing the proliferation of KGN cells treated with 10 μM LY294002 or SC79 after 48 h of FABP5 overexpression or knockdown, as determined by CCK8 assays.* represents the comparison between the EV-ctrl and myc-FABP5 or siNC and siFABP5 groups; # represents the comparison between the myc-FABP5 and myc-FABP5 + LY294002 or siFABP5 and siFABP5 + SC79 groups; D, F Immunofluorescence staining of Ki67 in KGN cells treated with 10 μM LY294002 or SC79 for 48 h after FABP5 was overexpressed or knocked down.Scale bar, 100 μm.E, G Statistical analysis of Ki67-positive cells.Five fields of view were randomly selected.*P < 0.05, **P < 0.01, ***P < 0.001.##P < 0.01, ###P < 0.01

Fig. 7 FABP5
Fig. 7 FABP5 accelerates fatty acid synthesis in KGN cells by activating PI3K-AKT signaling (A, C) Nile red staining of KGN cells treated with 10 μM LY294002 or SC79 for 48 h after 48 h of FABP5 overexpression or knockdown.The red signal indicates the formation of lipid droplets.All the samples were also stained with DAPI.Scale bar, 100 μm.B, D Statistical analysis of the number of lipid droplets.Five fields of view were randomly selected.E, G Bar plot showing ACSL1, GPAM1, LPIN1 and DGAT2 mRNA expression in KGN cells treated with 10 μM LY294002 or SC79 for 48 h after FABP5 overexpression or knockdown.A two-tailed unpaired t test was used for all the statistical analyses.*P < 0.05, **P < 0.01, ***P < 0.001.

Table 1
qRT-PCR primers sequencesData were analyzed by using the 2 −ΔΔCt relative quantitative method with human or mouse 18 s rRNA as an internal control and A two-tailed unpaired t-test was used for statistical analysis